00443nas a2200157 4500008004100000245004800041210004000089490000800129100001500137700001900152700001600171700001700187700001600204700001600220856004900236 2020 eng d00aIs the Protein Dynamical Transition useful?0 aProtein Dynamical Transition useful0 v1181 aSharma, A.1 aGeorge, D., K.1 aCrossen, K.1 aMcKinney, J.1 aKerfeld, C.1 aMarkelz, A. uhttps://markelz.physics.buffalo.edu/node/28201881nas a2200181 4500008004100000245009900041210006900140260001600209520131100225100001701536700001501553700001301568700001501581700002201596700001601618700001601634856004901650 2020 eng d00aStabilization of Terahertz Vibrational Modes in Illuminated Orange Carotenoid Protein Crystals0 aStabilization of Terahertz Vibrational Modes in Illuminated Oran aBuffalo, NY3 a
Orange carotenoid protein (OCP) controls efficiency of the phycobilisome (PBS), the light harvesting antenna in cyanobacteria, to prevent oxidative damage. The OCP switches from resting state to photo protective state with intense blue light illumination. Questions persist as to why OCPR interaction increases with the PBS over that with the OCPO. Here we examine the role of long-range intramolecular vibrations within OCP. Using Stationary Sample Anisotropic Terahertz Microscopy (SSATM) we measure changes in the intramolecular vibrations with photo switching. We report the first observation of switching in the intramolecular vibrations with photoexcitation. Results suggest that there is a stiffening of the molecule in the photo protective state. This increase in structural stability may enhance the interaction with the PBS change in OCP interaction with PBS. In low light, carotenoid bound OCP appears orange (OCP o ) and is inactive. Illumination by strong light converts OCP to the active, red (OCPR) state, which interacts with the PBS. A comparison of anisotropic THz microscopy measurements of dark adapted (OCP o ) and illuminated OCP crystals indicate differences in their vibrational modes that may be important for OCP-PBS interactions.
1 aMcKinney, J.1 aSharma, A.1 aDeng, Y.1 aGeorge, D.1 aLechno-Yossef, S.1 aKerfeld, C.1 aMarkelz, A. uhttps://markelz.physics.buffalo.edu/node/54401100nas a2200169 4500008004500000020002200045245011300067210006900180260001900249520052500268100001700793700001700810700001500827700001900842700002000861856004900881 2019 Engldsh a978-1-5386-8285-200aAnisotropic Terahertz Microscopy of Protein Collective Vibrations: Crystal Symmetry and Hydration Dependence0 aAnisotropic Terahertz Microscopy of Protein Collective Vibration aNew YorkbIeee3 aA stationary sample anisotropic terahertz microscopy technique is used to characterize the intramolecular vibrations for lysozyme. Tetragonal and triclinic crystals are compared. We find excellent reproducibility within a single crystal symmetry group. Several resonant bands are present for both symmetry groups, indicating they originate with the intramolecular vibrations and not crystal lattice phonons. Bands become more pronounced and higher frequency resonant bands begin to emerge with slight dehydration.
1 aMcKinney, J.1 aDeng, Y., T.1 aSharma, A.1 aGeorge, D., K.1 aMarkelz, A., G. uhttps://markelz.physics.buffalo.edu/node/23500524nas a2200169 4500008004500000020001400045245008200059210006900141260000800210300001400218490000800232653001500240100001700255700001700272700001600289856004900305 2019 Engldsh a0006-349500aDeuteration and Inhibitor Binding Dependence of Protein Collective Vibrations0 aDeuteration and Inhibitor Binding Dependence of Protein Collecti cFeb a488A-488A0 v11610aBiophysics1 aDeng, Y., T.1 aMcKinney, J.1 aMarkelz, A. uhttps://markelz.physics.buffalo.edu/node/24800492nas a2200145 4500008004100000245008500041210006900126260001200195100001300207700001700220700001300237700001900250700002000269856005700289 2019 eng d00aProtein Intramolecular Motions with Deuteration and Inhibitor Binding Dependence0 aProtein Intramolecular Motions with Deuteration and Inhibitor Bi c03/20191 aDeng, Y.1 aMcKinney, J.1 aRomo, T.1 aGrossfield, A.1 aMarkelz, A., G. uhttps://meetings.aps.org/Meeting/MAR19/Session/R63.302599nas a2200205 4500008004500000020001400045245005800059210005800117260000800175300001400183490000800197520204200205653001502247100001702262700001702279700001302296700001902309700001602328856004902344 2019 Engldsh a0006-349500aSpectral Assignment of Lysozyme Collective Vibrations0 aSpectral Assignment of Lysozyme Collective Vibrations cFeb a564A-564A0 v1163 a