03325nas a2200145 4500008004100000245008500041210006900126260001200195520283400207100002103041700001903062700001303081700002003094856006503114 2021 eng d00aAnisotropic Terahertz Microscopy of Lysozyme in Different CrystalLattice Systems0 aAnisotropic Terahertz Microscopy of Lysozyme in Different Crysta c02/20213 a
Long-range vibrational modes of proteins at terahertz (THz) frequencies havebeen associated with protein function and allosteric control. The characteriza-tion of these motions has been challenging due to energy overlap with waterabsorption and a large vibrational density of states. Recently it has been demon-strated both experimentally and theoretically that vibrational bands can be iso-lated using stationary sample anisotropic terahertz microscopy (SSTAM) fororiented samples, typically realized using protein crystals [1, 2]. In those earlymeasurements, inhibitor binding contrast was demonstrated for high symmetrytetragonal crystals. While high symmetry crystals are ideal for structural deter-minations, they can limit the types of vibrations observable in the ATM mea-surements. Here we show a survey of ATM measurements of triclinic,monoclinic and tetragonal crystals, demonstrating the unique signaturesobservable for the different symmetry groups, leading to a more completedetermination of the vibrational hot spots that may contribute to enzymatic ef-ficiency. The SSATM spectra indicate the presence of conserved vibrationalmodes near 40 cm-1 and 55 cm-1 for CEWL in triclinic, monoclinic and tetrag-onal lattice systems respectively. For CEWL in the monoclinic lattice system, aprominent band at 20cm1was consistently observed in the SSATM spectrabut not in the triclinic or tetragonal systems. The conserved bands may repre-sent vibrational modes that are unperturbed by crystal contact forces while thedifferences may be related to unique molecular orientation in different crystalsystems.
1.Niessen, K., Y. Deng, and A.G. Markelz,Near-field THz micropo-larimetry.Opt Express, 2019.27(20): p. 28036-28047.
2.Romo, T.D., A.Grossfield, and A.G. MarkelzPersistent Protein Motions in a RuggedEnergy Landscape Revealed by Normal Mode Ensemble Analysis. AcceptedJournal of Chemical Information and Modeling, 2020.
1 aMcKinney, J., A.1 aGeorge, D., K.1 aDeng, Y.1 aMarkelz, A., G. uhttps://www.cell.com/biophysj/fulltext/S0006-3495(20)31879-801100nas a2200169 4500008004500000020002200045245011300067210006900180260001900249520052500268100001700793700001700810700001500827700001900842700002000861856004900881 2019 Engldsh a978-1-5386-8285-200aAnisotropic Terahertz Microscopy of Protein Collective Vibrations: Crystal Symmetry and Hydration Dependence0 aAnisotropic Terahertz Microscopy of Protein Collective Vibration aNew YorkbIeee3 aA stationary sample anisotropic terahertz microscopy technique is used to characterize the intramolecular vibrations for lysozyme. Tetragonal and triclinic crystals are compared. We find excellent reproducibility within a single crystal symmetry group. Several resonant bands are present for both symmetry groups, indicating they originate with the intramolecular vibrations and not crystal lattice phonons. Bands become more pronounced and higher frequency resonant bands begin to emerge with slight dehydration.
1 aMcKinney, J.1 aDeng, Y., T.1 aSharma, A.1 aGeorge, D., K.1 aMarkelz, A., G. uhttps://markelz.physics.buffalo.edu/node/235