TY - Generic T1 - Probing the Stability of Fluorescent Proteins by Terahertz Spectroscopy T2 - 2014 39th International Conference on Infrared, Millimeter, and Terahertz Waves Y1 - 2014 A1 - Xu, M. Y. A1 - George, D. K. A1 - Jimenez, R. A1 - Markelz, A. G. KW - dynamics AB -

The higher transmission through tissues of long wavelength light motivates the development of fluorescent proteins with excitation shifted to the red. However red fluorescent proteins (RFPs) are more susceptible to photobleaching than their shorter wavelength counterparts. In particular RFPs are more susceptible to photobleaching [1]. A possible reason for this is a decrease in the structural stability of the beta barrel. Measurements of structural stability include atomic root mean squared displacement <x(2)> measured by the X-ray B-factor and neutron quasi elastic scattering. To date, X-ray measurements of RFP's do not indicate a structural stability change and systematic scattering studies have not been performed. Using THz dielectric response we examine if the picosecond structural flexibility decreases with increasing FP stability.

JF - 2014 39th International Conference on Infrared, Millimeter, and Terahertz Waves T3 - International Conference on Infrared Millimeter and Terahertz Waves PB - Ieee CY - New York SN - 978-1-4799-3877-3 N1 - ISI Document Delivery No.: BF0IL
Times Cited: 0
Cited Reference Count: 7
Cited References:
Dean KM, 2011, BIOPHYS J, V101, P961, DOI 10.1016/j.bpj.2011.06.055
He YF, 2011, BIOPHYS J, V100, P1058, DOI 10.1016/j.bpj.2010.12.3731
Helms V, 2007, CHEMPHYSCHEM, V8, P23, DOI 10.1002/cphc.200600298
Leu BM, 2008, BIOPHYS J, V95, P5874, DOI 10.1529/biophysj.108.138198
Markelz AG, 2007, CHEM PHYS LETT, V442, P413, DOI 10.1016/j.cplett.2007.05.080
Nagy A, 2004, THERMOCHIM ACTA, V410, P161, DOI 10.1016/S0040-6031(03)00397-6
Zaccai G, 2000, SCIENCE, V288, P1604, DOI 10.1126/science.288.5471.1604
Xu, Mengyang George, D. K. Jimenez, R. Markelz, A. G.
Irmmw-thz
Proceedings Paper
39th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz)
Sep 14-19, 2014
Tucson, AZ
THORLABS, Tydex, TOPTICA Photon, Bruker, Gentec EO, Lake Shore Cryotron, Ekspla, Zomega, TeraSense, Insight Product, Emcore, QMC Instruments, TeraView, NeaSpec, Advantest, MenloSystems, Traycer, Microtech Instruments Inc, LongWave Photon, Virginia Diodes Inc, ASU, MTT S, Journal Infrared Millimeter & Tera Hertz Waves, Tera Hertz Sci & Technol, Army Res Off
George, Deepu/J-9882-2014
George, Deepu/0000-0003-0021-0705
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