TY - Generic T1 - Anisotropic Absorption Measurements Reveal Protein Dynamical Transition in Intramolecular Vibrations T2 - 2016 41st International Conference on Infrared, Millimeter, and Terahertz Waves Y1 - 2016 A1 - Xu, M. Y. A1 - Niessen, K. A1 - Michki, N. A1 - Deng, Y. T. A1 - Snell, E. A1 - Markelz, A. G. AB -

Modeling has predicted that intramolecular structural vibrations enables proteins to access functionally important structural change. We show that the vibrational density of states and the isotropic absorption in the terahertz range are only weakly dependent on the protein functional state for several bench marking proteins. At the same time the direction of motions changes dramatically with functional state and with a resulting impact on the anisotropic absorption. Our anisotropic THz microscopy (ATM) measurements confirm this sensitivity. Here we apply the technique to the question of whether the protein dynamical transition (DT) is important to protein function. We find a strong anisotropic resonance at 70 cm(-1) rapidly increases in strength at temperatures above the DT. As these intramolecular vibrations enable protein structure to change conformation, the results suggest function will cease below DT for those proteins that require large scale conformational change.

JF - 2016 41st International Conference on Infrared, Millimeter, and Terahertz Waves T3 - International Conference on Infrared Millimeter and Terahertz Waves PB - Ieee CY - New York SN - 978-1-4673-8485-8 N1 - ISI Document Delivery No.: BG7KC
Times Cited: 0
Cited Reference Count: 4
Cited References:
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Niessen K., 2015, BIOPHYSICAL REV
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Xu, Mengyang Niessen, Katherine Michki, Nigel Deng, Yanting Snell, Edward Markelz, A. G.
Irmmw-thz
Proceedings Paper
41st International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz)
Sep 25-30, 2016
Copenhagen, DENMARK
DTU, IEEE, QMC Instruments, Danish Ctr Laser Infrastructure, DTU Fotonik, Dept Photon Engn, ARL, Carl Sberg Fdn, AF Off Sci Res, Tech Univ Denmark, IEEE Microwave Theory & Tech Soc, Azpect Photon, Ekspla, Hubner HF Syst Engn, I2S, Laser Quantum, Menlo Syst, Neaspec, Springer, TeraView, Virginia Diodes
Snell, Edward/G-2055-2018
Snell, Edward/0000-0001-8714-3191
345 e 47th st, new york, ny 10017 usa
2162-2027 ER - TY - Generic T1 - Measurements and Calculations of Protein Intramolecular Vibrations in the THz Range T2 - 2014 39th International Conference on Infrared, Millimeter, and Terahertz Waves Y1 - 2014 A1 - Niessen, K. A. A1 - Snell, E. A1 - Markelz, A. G. KW - charmm AB -

We report the calculations and measurements of intramolecular vibrational modes and their dependence on inhibitor binding in the THz range. We see an increase in anisotropic THz absorption at low frequency with inhibitor binding in both the measurements and calculations. This surprising result suggests an increase in flexibility with binding. We will discuss the possible reasons for this discrepancy.

JF - 2014 39th International Conference on Infrared, Millimeter, and Terahertz Waves T3 - International Conference on Infrared Millimeter and Terahertz Waves PB - Ieee CY - New York SN - 978-1-4799-3877-3 N1 - ISI Document Delivery No.: BF0IL
Times Cited: 0
Cited Reference Count: 13
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Niessen, Katherine A. Snell, Edward Markelz, A. G.
Irmmw-thz
Proceedings Paper
39th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz)
Sep 14-19, 2014
Tucson, AZ
THORLABS, Tydex, TOPTICA Photon, Bruker, Gentec EO, Lake Shore Cryotron, Ekspla, Zomega, TeraSense, Insight Product, Emcore, QMC Instruments, TeraView, NeaSpec, Advantest, MenloSystems, Traycer, Microtech Instruments Inc, LongWave Photon, Virginia Diodes Inc, ASU, MTT S, Journal Infrared Millimeter & Tera Hertz Waves, Tera Hertz Sci & Technol, Army Res Off
Snell, Edward/G-2055-2018
Snell, Edward/0000-0001-8714-3191
345 e 47th st, new york, ny 10017 usa
2162-2027 ER - TY - Generic T1 - Measuring phonons in protein crystals T2 - Ultrafast Phenomena and Nanophotonics Xvii Y1 - 2013 A1 - Acbas, G. A1 - Niessen, K. A. A1 - George, D. K. A1 - Snell, E. A1 - Markelz, A. G. ED - Betz, M. ED - Elezzabi, A. Y. ED - Song, J. J. ED - Tsen, K. T. KW - correlated motions KW - dynamics KW - mode KW - molecular crystals KW - molecular vibrations KW - normal modes KW - phonons KW - protein dynamics KW - spectroscopy KW - Terahertz AB -

Using Terahertz near field microscopy we find orientation dependent narrow band absorption features for lysozyme crystals. Here we discuss identification of protein collective modes associated with the observed features. Using normal mode calculations we find good agreement with several of the measured features, suggesting that the modes arise from internal molecular motions and not crystal phonons. Such internal modes have been associated with protein function.

JF - Ultrafast Phenomena and Nanophotonics Xvii T3 - Proceedings of SPIE PB - Spie-Int Soc Optical Engineering CY - Bellingham VL - 8623 SN - 978-0-8194-9392-7 N1 - ISI Document Delivery No.: BGG42
Times Cited: 0
Cited Reference Count: 5
Cited References:
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Planken PCM, 2011, J INFRARED MILLIM TE, V32, P975, DOI 10.1007/s10762-011-9824-3
Acbas, Gheorghe Niessen, Katherine A. George, Deepu K. Snell, Edward Markelz, A. G.
Proceedings Paper
Conference on Ultrafast Phenomena and Nanophotonics XVII
Feb 03-06, 2013
San Francisco, CA
SPIE, Femtolasers Inc
Snell, Edward/G-2055-2018; George, Deepu/J-9882-2014
Snell, Edward/0000-0001-8714-3191; George, Deepu/0000-0003-0021-0705; Markelz, Andrea/0000-0003-0443-4319
National Science Foundation MRI2 [DBI2959989]
We thank the National Science Foundation MRI2 grant DBI2959989 for support.
1000 20th st, po box 10, bellingham, wa 98227-0010 usa
0277-786x
862305 ER - TY - Generic T1 - Orientation Sensitive Terahertz Resonances Observed in Protein Crystals T2 - 2012 37th International Conference on Infrared, Millimeter, and Terahertz Waves Y1 - 2012 A1 - Acbas, G. A1 - Snell, E. A1 - Markelz, A. G. KW - dynamics KW - mode AB -

A method is presented for measuring anisotropic THz response for small crystals, Crystal Anisotropy Terahertz Microscopy (CATM). Sucrose CATM measurements find the expected anisotropic phonon resonances. CATM measurements of protein crystals find the expected broadband water absorption is suppressed and strong orientation and hydration dependent resonant features.

JF - 2012 37th International Conference on Infrared, Millimeter, and Terahertz Waves T3 - International Conference on Infrared Millimeter and Terahertz Waves PB - Ieee CY - New York SN - 978-1-4673-1597-5 N1 - ISI Document Delivery No.: BJT74
Times Cited: 1
Cited Reference Count: 15
Cited References:
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Tych KM, 2011, J APPL CRYSTALLOGR, V44, P129, DOI 10.1107/S0021889810043372
Acbas, Gheorghe Snell, Edward Markelz, A. G.
Irmmw-thz
Proceedings Paper
37th International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz)
Sep 23-28, 2012
Univ Wollongong, Wollongong, AUSTRALIA
IEEE, USN, Off Naval Res Sci & Technol, ETRI, UOW, Sch Engn Phys, Ctr Ultrahigh Bandwidth Devices Opt Syst, Victoria Suntech Adv Solar Facil, Swinburne, Ctr Micro Photon, Edinburgh Photon, Tydex, TRAS Inc, Inst Photon & Opt Sci, LakeShore, Australian Synchrotron, CSIRO, Univ Wollongong, Inst Superconducting & Elect Mat, Ctr Med Radiat Phys, Univ Sydney, IEEE Microwave Theory & Tech Soc
Snell, Edward/G-2055-2018
Snell, Edward/0000-0001-8714-3191
345 e 47th st, new york, ny 10017 usa
2162-2027 ER -