TY - JOUR T1 - Modulated orientation-sensitive terahertz spectroscopy JF - Photonics Research Y1 - 2016 A1 - Singh, R. A1 - George, D. K. A1 - Bae, C. J. A1 - Niessen, K. A. A1 - Markelz, A. G. KW - absorption-spectroscopy KW - dynamics KW - enzyme catalysis KW - low-frequency modes KW - lysozyme KW - neutron-scattering KW - Optics KW - perspective KW - polarization modulation KW - proteins KW - water AB -

Intramolecular vibrations of large macromolecules reside in the terahertz range. In particular, protein vibrations are closely spaced in frequency, resulting in a nearly continuous vibrational density of states. This density of vibrations interferes with the identification of specific absorption lines and their subsequent association with specific functional motions. This challenge is compounded with the absorption being dominated by the solvent and local relaxational motions. A strategy for removing the isotropic relaxational loss and isolating specific vibrations is to use aligned samples and polarization-sensitive measurements. Here, we demonstrate a technique to rapidly attain the anisotropic resonant absorbance using terahertz time domain spectroscopy and a spinning sample. The technique, modulated orientation-sensitive terahertz spectroscopy (MOSTS), has a nonzero signal only for anisotropic samples, as demonstrated by a comparison between a silicon wafer and a wire grid polarizer. For sucrose and oxalic acid molecular crystals, the MOSTS response is in agreement with modeled results for the intermolecular vibrations. Further, we demonstrate that, even in the presence of a large relaxational background, MOSTS isolates underlying vibrational resonances. (C) 2016 Chinese Laser Press

VL - 4 SN - 2327-9125 N1 - ISI Document Delivery No.: DM9BJ
Times Cited: 6
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Singh, Rohit George, Deepu Koshy Bae, Chejin Niessen, K. A. Markelz, A. G.
George, Deepu/J-9882-2014
George, Deepu/0000-0003-0021-0705
National Science Foundation (NSF)National Science Foundation (NSF) [DBI2959989]; University at Buffalo (UB) [1126960-1-68255]; Los Alamos National Laboratory (LANL)United States Department of Energy (DOE)Los Alamos National Laboratory [1125895-1-71842]; Direct For Biological SciencesNational Science Foundation (NSF)NSF - Directorate for Biological Sciences (BIO) [1556359] Funding Source: National Science Foundation; Div Of Biological InfrastructureNational Science Foundation (NSF)NSF - Directorate for Biological Sciences (BIO) [1556359] Funding Source: National Science Foundation
National Science Foundation (NSF) (DBI2959989); University at Buffalo (UB) (Holm Fund 1126960-1-68255); Los Alamos National Laboratory (LANL) (Molecular Crystal Studies 1125895-1-71842).
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Optical soc amer
Washington JO - Photonics Res.Photonics Res. ER - TY - Generic T1 - Measurements and Calculations of Protein Intramolecular Vibrations in the THz Range T2 - 2014 39th International Conference on Infrared, Millimeter, and Terahertz Waves Y1 - 2014 A1 - Niessen, K. A. A1 - Snell, E. A1 - Markelz, A. G. KW - charmm AB -

We report the calculations and measurements of intramolecular vibrational modes and their dependence on inhibitor binding in the THz range. We see an increase in anisotropic THz absorption at low frequency with inhibitor binding in both the measurements and calculations. This surprising result suggests an increase in flexibility with binding. We will discuss the possible reasons for this discrepancy.

JF - 2014 39th International Conference on Infrared, Millimeter, and Terahertz Waves T3 - International Conference on Infrared Millimeter and Terahertz Waves PB - Ieee CY - New York SN - 978-1-4799-3877-3 N1 - ISI Document Delivery No.: BF0IL
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Niessen, Katherine A. Snell, Edward Markelz, A. G.
Irmmw-thz
Proceedings Paper
39th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz)
Sep 14-19, 2014
Tucson, AZ
THORLABS, Tydex, TOPTICA Photon, Bruker, Gentec EO, Lake Shore Cryotron, Ekspla, Zomega, TeraSense, Insight Product, Emcore, QMC Instruments, TeraView, NeaSpec, Advantest, MenloSystems, Traycer, Microtech Instruments Inc, LongWave Photon, Virginia Diodes Inc, ASU, MTT S, Journal Infrared Millimeter & Tera Hertz Waves, Tera Hertz Sci & Technol, Army Res Off
Snell, Edward/G-2055-2018
Snell, Edward/0000-0001-8714-3191
345 e 47th st, new york, ny 10017 usa
2162-2027 ER - TY - Generic T1 - Measuring phonons in protein crystals T2 - Ultrafast Phenomena and Nanophotonics Xvii Y1 - 2013 A1 - Acbas, G. A1 - Niessen, K. A. A1 - George, D. K. A1 - Snell, E. A1 - Markelz, A. G. ED - Betz, M. ED - Elezzabi, A. Y. ED - Song, J. J. ED - Tsen, K. T. KW - correlated motions KW - dynamics KW - mode KW - molecular crystals KW - molecular vibrations KW - normal modes KW - phonons KW - protein dynamics KW - spectroscopy KW - Terahertz AB -

Using Terahertz near field microscopy we find orientation dependent narrow band absorption features for lysozyme crystals. Here we discuss identification of protein collective modes associated with the observed features. Using normal mode calculations we find good agreement with several of the measured features, suggesting that the modes arise from internal molecular motions and not crystal phonons. Such internal modes have been associated with protein function.

JF - Ultrafast Phenomena and Nanophotonics Xvii T3 - Proceedings of SPIE PB - Spie-Int Soc Optical Engineering CY - Bellingham VL - 8623 SN - 978-0-8194-9392-7 N1 - ISI Document Delivery No.: BGG42
Times Cited: 0
Cited Reference Count: 5
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Acbas, Gheorghe Niessen, Katherine A. George, Deepu K. Snell, Edward Markelz, A. G.
Proceedings Paper
Conference on Ultrafast Phenomena and Nanophotonics XVII
Feb 03-06, 2013
San Francisco, CA
SPIE, Femtolasers Inc
Snell, Edward/G-2055-2018; George, Deepu/J-9882-2014
Snell, Edward/0000-0001-8714-3191; George, Deepu/0000-0003-0021-0705; Markelz, Andrea/0000-0003-0443-4319
National Science Foundation MRI2 [DBI2959989]
We thank the National Science Foundation MRI2 grant DBI2959989 for support.
1000 20th st, po box 10, bellingham, wa 98227-0010 usa
0277-786x
862305 ER -