<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>47</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">McKinney, J. A.</style></author><author><style face="normal" font="default" size="100%">George, D. K.</style></author><author><style face="normal" font="default" size="100%">Deng, Y.</style></author><author><style face="normal" font="default" size="100%">Markelz, A. G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anisotropic Terahertz Microscopy of Lysozyme in Different CrystalLattice Systems</style></title><secondary-title><style face="normal" font="default" size="100%">Biophysical Journal</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">02/2021</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.cell.com/biophysj/fulltext/S0006-3495(20)31879-8</style></url></web-urls></urls><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Long-range vibrational modes of proteins at terahertz (THz) frequencies havebeen associated with protein function and allosteric control. The characteriza-tion of these motions has been challenging due to energy overlap with waterabsorption and a large vibrational density of states. Recently it has been demon-strated both experimentally and theoretically that vibrational bands can be iso-lated using stationary sample anisotropic terahertz microscopy (SSTAM) fororiented samples, typically realized using protein crystals [1, 2]. In those earlymeasurements, inhibitor binding contrast was demonstrated for high symmetrytetragonal crystals. While high symmetry crystals are ideal for structural deter-minations, they can limit the types of vibrations observable in the ATM mea-surements. Here we show a survey of ATM measurements of triclinic,monoclinic and tetragonal crystals, demonstrating the unique signaturesobservable for the different symmetry groups, leading to a more completedetermination of the vibrational hot spots that may contribute to enzymatic ef-ficiency. The SSATM spectra indicate the presence of conserved vibrationalmodes near 40 cm&lt;sup&gt;-1&lt;/sup&gt; and 55 cm&lt;sup&gt;-1&lt;/sup&gt; for CEWL in triclinic, monoclinic and tetrag-onal lattice systems respectively. For CEWL in the monoclinic lattice system, aprominent band at 20cm1was consistently observed in the SSATM spectrabut not in the triclinic or tetragonal systems. The conserved bands may repre-sent vibrational modes that are unperturbed by crystal contact forces while thedifferences may be related to unique molecular orientation in different crystalsystems.&lt;/p&gt;

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&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;1.Niessen, K., Y. Deng, and A.G. Markelz,&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;Near-field THz micropo-&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;larimetry.&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;Opt Express, 2019.&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;27&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;(20): p. 28036-28047.&lt;/span&gt;&lt;/p&gt;

&lt;p&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;2.Romo, T.D., A.&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;Grossfield, and A.G. Markelz&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;Persistent Protein Motions in a Rugged&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;Energy Landscape Revealed by Normal Mode Ensemble Analysis&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;. Accepted&lt;/span&gt;&lt;span dir=&quot;ltr&quot; style=&quot;font-family:serif; font-size:13.2837px&quot;&gt;Journal of Chemical Information and Modeling, 2020.&lt;/span&gt;&lt;/p&gt;
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