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THz Spectroscopy at the University at Buffalo

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Home » 01 | Protocols » Expression & Purification Guide

3. Lab Protocols

Lab protocols are designed to facilitate good practices in the lab for a variety of basic procedures.

  • 3.01. Calibrating the pH Meter
  • 3.02. Preparing Buffer Solutions
  • 3.03. Filtering Solutions with a Syringe
  • 3.04. Preparing Growth
  • 3.05. Pouring Agar Plates
  • 3.06. Streaking Agar Plates with E. Coli
  • 3.07. Preparing Glycerol Stocks
  • 3.08. Inoculating Liquid Cultures with an Autoclaved Toothpick
  • 3.09. Inoculating Liquid with a Loop
  • 3.10. Preparing 2 ml Liquid Cultures
  • 3.11. Inducing 1 ml Culture with IPTG
  • 3.12. Measuring OD600 with Nanodrop
  • 3.13. Growing Expression Cultures
  • 3.14. Harvesting Cells from an Overnight Culture
  • 3.15. Handcasting Polyacrylamide Gels
  • 3.16. Running Polyacrylamide Gels
  • 3.17. Staining Destaining Gels
  • 3.18. Testing a Glycerol Stock for Protein Over-Expression
‹ 2.3. Communicate up 3.01. Calibrating the pH Meter ›

Book navigation

  • 01 | Protocols
    • Cleaning SSATM Cups
    • Expression & Purification Guide
      • 1. Lab Equipment
      • 2. Lab Practices
      • 3. Lab Protocols
        • 3.01. Calibrating the pH Meter
        • 3.02. Preparing Buffer Solutions
        • 3.03. Filtering Solutions with a Syringe
        • 3.04. Preparing Growth
        • 3.05. Pouring Agar Plates
        • 3.06. Streaking Agar Plates with E. Coli
        • 3.07. Preparing Glycerol Stocks
        • 3.08. Inoculating Liquid Cultures with an Autoclaved Toothpick
        • 3.09. Inoculating Liquid with a Loop
        • 3.10. Preparing 2 ml Liquid Cultures
        • 3.11. Inducing 1 ml Culture with IPTG
        • 3.12. Measuring OD600 with Nanodrop
        • 3.13. Growing Expression Cultures
        • 3.14. Harvesting Cells from an Overnight Culture
        • 3.15. Handcasting Polyacrylamide Gels
        • 3.16. Running Polyacrylamide Gels
        • 3.17. Staining Destaining Gels
        • 3.18. Testing a Glycerol Stock for Protein Over-Expression
      • 4. Resources
    • Procedure for Face Indexing
  • 02 | The Optics Library