- Remove the inner chamber and pour the bu er solution into the outer chamber.
- Remove the glass cassette from the inner chamber.
- Using a plastic scraper, carefully separate the planes of glass and remove the gel from the glass.
- Use the scraper to chop o the stacking gel.
- Use the scraper to chop o the top left corner of the resolving gel.
- Place the gel in a container that is large enough to allow the gel to lie flat.
- Pour enough stain solution to cover the gel.
- Allow the gel to stain overnight on a rocker.
- Save the stain solution.
- Rinse the gel and container with deionized water.
- Place the gel and a rolled up piece of paper towel in the container.
- Pour enough destain solution into the container to cover the gel.
- Wait until the lines on the gel are visible, then take a picture of the gel.
https://www.sciencedirect.com/science/article/pii/B9780124201194000136#f0005 |