3.11. Inducing 1 ml Culture with IPTG

This process is used as a positive control to ensure that the desired protein is actually being produced by the bacteria. Therefore, an excess of IPTG is used to guarantee expression. The protein from this process is not to be analyzed by gel electrophoresis.

1. Transfer 1 ml of bacteria from the culture tube to a sterile culture tube.
2. Add IPTG to a concentration of 1 mM.
3. Repeat with remaining cultures.
4. Incubate cultures in a shaking incubator.