3.09. Inoculating Liquid with a Loop

1. Sterilize the loop with an open flame. Make sure to sterilize the neck of the loop with the flame as well.
2. Touch the loop to the rst colony. Make sure that the loop does not touch any other colonies.
3. Uncap the first culture tube.
4. Touch the loop to the liquid in the culture tube. Try not to touch the sides of the tube with the loop. If possible, touch the loop to a drop on the side of the tube rather than the liquid at the bottom of the tube.
5. Remove the loop from the tube and recap the tube.
6. Holding the cap in place, shake the tube.
7. Repeat steps 1-6 with the remaining colonies.

Note: When inoculating cultures from glycerol stocks, the glycerol stock should be kept in a cold block when they are not in the freezer.

Figure 4. Glycerol stock in cold block about to be used to inoculate a sterile flask.