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Home » 01 | Protocols » Expression & Purification Guide » 3. Lab Protocols

3.12. Measuring OD600 with Nanodrop

  1. Turn on the nanodrop. The switch is in the back.
  2. Set nanodrop to OD600
  3. Lift the arm of the nanodrop. Figure 5a.
  4. Insert a cuvette containing sterile LB media, and lower the arm.
  5. Click the blank button.
  6. Remove the blank cuvette and insert the cuvette to be measured.
  7. Click the measure button.
  8. Record the OD600.

(a)

(b)

Figure 5

 

 

‹ 3.11. Inducing 1 ml Culture with IPTG up 3.13. Growing Expression Cultures ›

Book navigation

  • 01 | Protocols
    • Cleaning SSATM Cups
    • Expression & Purification Guide
      • 1. Lab Equipment
      • 2. Lab Practices
      • 3. Lab Protocols
        • 3.01. Calibrating the pH Meter
        • 3.02. Preparing Buffer Solutions
        • 3.03. Filtering Solutions with a Syringe
        • 3.04. Preparing Growth
        • 3.05. Pouring Agar Plates
        • 3.06. Streaking Agar Plates with E. Coli
        • 3.07. Preparing Glycerol Stocks
        • 3.08. Inoculating Liquid Cultures with an Autoclaved Toothpick
        • 3.09. Inoculating Liquid with a Loop
        • 3.10. Preparing 2 ml Liquid Cultures
        • 3.11. Inducing 1 ml Culture with IPTG
        • 3.12. Measuring OD600 with Nanodrop
        • 3.13. Growing Expression Cultures
        • 3.14. Harvesting Cells from an Overnight Culture
        • 3.15. Handcasting Polyacrylamide Gels
        • 3.16. Running Polyacrylamide Gels
        • 3.17. Staining Destaining Gels
        • 3.18. Testing a Glycerol Stock for Protein Over-Expression
      • 4. Resources
    • Procedure for Face Indexing
  • 02 | The Optics Library