Place the glass cassette into the electrophoresis chamber with the short piece of glass facing inwards.
If only one gel is being ran, place a bu er dam on the other side of the electrophoresis chamber. If two gels are being ran, place the second gel on the other side of electrophoresis chamber with the short piece of glass facing inwards.
Using the green clamps, lock the glass cassttes/dam into the chamber.
Prepare 750 ml of 1X SDS-PAGE Buffer.
Place the inner chamber into the outer chamber making sure to align the red side with the red side and the black side with the black side.
Fill the inner chamber with 1X SDS-PAGE bu er, so that the bu er is above the top of the short plate, but below the top of the tall plate. Make sure that the inner chamber is not leaking. If it is, dump the buffer into the outer chamber then reassemble and refill the inner chamber.
Pour the remainder of the buffer into the outer chamber.
Load each well with 10 μl of sample.
Place the lid on the electrophoresis chamber making sure to align the red side with the red side and the black side with the black side.
Place the leads into the power supply matching red to red and black to black.
Set the power supply to a constant voltage of 200 V.
Turn on the power supply. You should see bubbles in the inner chamber at this point.
Let the gel run until the tracking dye reaches the bottom of the glass cassette. This should take about 45-60 minutes.