- Prepare a culture tube with 2 ml of LB with antibiotics.
- Uncap the toothpick vial with your little finger.
- Tilt the vial so that it is sideways.
- Gently shake the toothpick vial so that some of the toothpicks are sticking out of the open end.
- Carefully remove a single toothpick with your pointer and index finger.
- Holding onto the toothpick, recap the vial.
- Gently touch the tip of the toothpick to the rst colony. Make sure that the tip does not touch any other colonies.
- Uncap the rst culture tube.
- Drop the toothpick into the rst culture tube.
- Recap the tube.
- Repeat steps 1-10 with the remaining colonies.
The following video provides a good explanation of this process (see below): https://www.youtube.com/watch?v=5XbLrXtWNOk
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